Mapping the Duodenal Crypt-Villus Transport Axis
نویسندگان
چکیده
منابع مشابه
Epithelial hedgehog signals pattern the intestinal crypt-villus axis.
Morphological development of the small intestinal mucosa involves the stepwise remodeling of a smooth-surfaced endodermal tube to form finger-like luminal projections (villi) and flask-shaped invaginations (crypts). These remodeling processes are orchestrated by instructive signals that pass bidirectionally between the epithelium and underlying mesenchyme. Sonic (Shh) and Indian (Ihh) hedgehog ...
متن کاملDifferential proteome analysis along jejunal crypt-villus axis in piglets.
The brush-border membrane (BBM) of enterocytes is responsible for the digestion and absorption of nutrients and ions in the small intestine. To identify the BBM proteins involved in epithelial cell maturation along the crypt-villus axis, enterocytes were sequentially isolated from the villus tip to the crypt of the jejunum from 21-day-old suckling piglets. After preparation of BBM vesicles, we ...
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A variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation of cells from the villus to the crypt has been reported in some of these papers, which allows the comparative study of terminally differentiated and proliferative cell phenotypes. However, these methods often involve the incubation of tissues at 37...
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BACKGROUND Reactive oxygen species (ROS) have been implicated in the turnover of epithelial cells in the rat intestine. The metabolism of ethanol generates ROS, which are implicated in cellular injury, but the levels of lipid peroxidation in intestine in chronic alcoholism are unknown. AIM To investigate the effects of ethanol ingestion on lipid peroxidation, and anti- and pro-oxidant enzyme ...
متن کاملHuman cell models to study small intestinal functions: recapitulation of the crypt-villus axis.
The intestinal epithelium is continuously and rapidly renewed by a process involving cell generation, migration, and differentiation, from the stem cell population located at the bottom of the crypt to the extrusion of the terminally differentiated cells at the tip of the villus. Because of the lack of normal human intestinal cell models, most of our knowledge about the regulation of human inte...
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ژورنال
عنوان ژورنال: Cellular and Molecular Gastroenterology and Hepatology
سال: 2018
ISSN: 2352-345X
DOI: 10.1016/j.jcmgh.2018.02.010